Now showing items 21-30 of 70
Evidence that isoniazid and ethanol induce the same microsomal cytochrome P-450 in rat liver, an isozyme homologous to rabbit liver cytochrome P-450 isozyme 3a
(Elsevier, 1986-05-01)
Cytochrome P-450j has been purified to electrophoretic homogeneity from hepatic microsomes of adult male rats administered ethanol and compared to the corresponding enzyme from isoniazid-treated rats. The enzymes isolated ...
Lectin binding studies on murine peritoneal cells: Physicochemical characterization of the binding of lectins from Datura stramonium, Evonymus europaea, and Griffonia simplicifolia to murine peritoneal cells
(Elsevier, 1986-01)
Purified 125I-labeled lectins from Datura stramonium, Evonymus europaea, and Griffonia simplicifolia (I-B4 isolectin) were used to analyze changes in the expression of carbohydrates on the surface of resident (PC) and ...
Ethanolaminephosphotransferase in rat lung: selectivity for endogenous and exogenous diacylglycerol
(Elsevier, 1988-05-02)
Ethanolaminephosphotransferase (EC 2.7.8.1) activity was determined in lung microsomes using diacylglycerols generated endogenously from [14C]glycerol 3-phosphate and different mixtures of fatty acids Ethanolaminephospho ...
Quantitation of oligosaccharides released by the [beta]-elimination reaction
(Elsevier, 1980-01-01)
A quantitative micromethod has been described for monitoring the rate and extent of the [beta]-elimination reaction as applied to O-glycosyl-glycoproteins utilizing alkaline tritiated borohydride. The procedure simultaneously ...
Immobilization and assay of low-molecular-weight phosphomannosyl receptor in multiwell plates
(Elsevier, 1987-10)
A novel sensitive binding assay for quantitation of a low-molecular-weight phosphomannosyl receptor (41-46 K) was devised. The receptor was immobilized by immunochemical means in the wells of polystyrene multiwell plates. ...
Bovine testicular [beta]-galactosidase: Purification of enzyme fractions that exhibit high affinity for phosphomannosyl receptors
(Elsevier, 1989-11-01)
An improved method is described for the preparation of bovine testicular [beta]-galactosidase that allows the isolation of enzyme fractions that bind avidly to phosphomannosyl receptors. The procedure permits removal of a ...
An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots
(Elsevier, 1983-07-01)
An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on thin-layer chromatography ...
Subcellular distribution and properties of acyl/alkyl dihydroxyacetone phosphate reductase in rodent livers
(Elsevier, 1986-03)
On subcellular fractionation, the enzyme acyl/alkyl dihydroxyacetone phosphate (DHAP) reductase (EC 1.1.1.101) in guinea pig and rat liver was found to be present in both the light mitochondrial (L) and microsomal fractions. ...
Radiometric assay for cytochrome P-450-catalyzed progesterone 16[alpha]-hydroxylation and determination of an apparent isotope effect
(Elsevier, 1987-08-01)
In the course of studies on the oxygenation of steroids by purified P-450 cytochromes, particularly rabbit liver microsomal cytochrome P-450 form 3b, a rapid and reliable radiometric assay has been devised for progesterone ...
Properties of the enzymes catalyzing the biosynthesis of lysophosphatidate and its ether analog in cultured fibroblasts from Zellweger syndrome patients and normal controls
(Elsevier, 1987-05-01)
The activities, properties, and steady-state kinetics of the five enzymes catalyzing the synthesis of 1-acyl- and 1-alkyl-sn-glycerol 3-phosphate in the cultured skin fibroblasts from Zellweger syndrome patients and normal ...