Now showing items 31-40 of 70
Cd2+ activation of -threonine dehydrogenase from Escherichia coli K-12
(Elsevier, 1988-11-23)
Homogeneous preparations of -threonine dehydrogenase (-threonine: NAD+ oxidoreductase, EC 1.1.1.103) from Escherichia coli K-12, after having been dialyzed against buffers containing Chelex-100 resin, have a basal level ...
Properties of the lectin from the hog peanut (Amphicarpaea bracteata)
(Elsevier, 1987-05-15)
An N-acetyl--galactosamine-specific lectin has been isolated from the two seed forms of the hog peanut (Amphicarpaea bracteata) using an affinity support containing the synthetic type A blood group trisaccharide ...
Stereospecific synthesis and enzyme studies of CDP-diacylglycerols
(Elsevier, 1982-09-14)
The fatty acid specificity of two enzymes that metabolize CDPdiacylglycerol, CDPdiacylglycerol hydrolase (EC 3.6.1.26) and CDPdiacylglycerol:inositol phosphatidyltransferase (EC 2.7.8.11), has been examined in guinea pig ...
Folylpolyglutamates as substrates and inhibitors of folate-dependent enzymes
(Elsevier, 1987)
The true intracellular substrates for folate-dependent enzymes are folylpolyglutamates. We have used measurements of the Ki values of folylpolyglutamate dead end inhibitors to assess the relative affinities of folate-dependent ...
Activation of CTP : Phosphocholine cytidylyltransferase in rat lung by fatty acids
(Elsevier, 1981-07-24)
CTP : phosphocholine cytidylyltransferase activity exists in both the microsome and cytosol fractions of adult lung, 36 and 59%, respectively. Although these enzyme activities are stimulated in vitro by added lipid activators ...
Bovine prothrombin does not contain [alpha]--galactopyranosyl groups
(Elsevier, 1980-02)
Using purified lectins (Bandeiraea simplicifolia I-B4 isolectin and the castor bean agglutinin) and glycosidases ([alpha]- and [beta]-galactosidases) we have shown that the terminal, non-reducing galactopyranosyl groups ...
Estimation of acyldihydroxyacetone phosphate and lysophosphatidate in animal tissues
(Elsevier, 1984-11-14)
Chemical and enzymatic methods have been developed to measure small quantities (10-8-10-10 mol) of acyldihydroxyacetone phosphate in animal tissues. Lipids extracted from tissue samples with acidic CHCl3/ methanol were ...
Solubilization and partial purification of dihydroxyacetone-phosphate acyltransferase from guinea pig liver
(Elsevier, 1983-10-01)
Dihydroxyacetone-phosphate:acyl coenzyme A acyltransferase (EC 2.3.1.42) was solubilized and partially purified from guinea pig liver crude peroxisomal fraction. The peroxisomal membrane was isolated after osmotic shock ...
Immunochemical evidence for a role of cytochrome P-450 in liver microsomal ethanol oxidation
(Elsevier, 1984-11-15)
Antibodies to cytochrome P-450 isozyme 3a, the ethanol-inducible isozyme in rabbit liver, were used to determine the role of this enzyme in the microsomal oxidation of alcohols and the p-hydroxylation of aniline. P-450 ...
Enzyme-linked lectin assay (ELLA): Use of alkaline phosphatase-conjugated Griffonia simplicifolia B4 isolectin for the detection of [alpha]--galactopyranosyl end groups
(Elsevier, 1983-04-15)
Alkaline phosphatase has been coupled to Griffonia simplicifolia I B4 isolectin using a one-step glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound ...