Now showing items 1-10 of 73
A specific radioimmunoassay for androstenedione with reduced bridge-binding
(Elsevier, 1984-09)
Antibody used in a steroid radioimmunoassay raised against a steroid hapten-carrier protein conjugate may recognize both the hapten and the chemical bridge to the protein. Use of the same bridge in the radioisotopic label ...
A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances
(Elsevier, 1984-03)
A thiobarbituric acid (TBA) reaction for measuring lipid peroxidation products was evaluated for interference by several ingredients commonly used in solutions to prepare or analyze tissue homogenates or subcellular ...
Mechanism and pharmacological specificity of dUTPase-mediated protection from DNA damage and cytotoxicity in human tumor cells
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1998-09)
Purpose : We have reported previously that the expression of E. coli dUTPase (dutE) can protect HT29 cells from 5-fluorodeoxyuridine (FdUrd)-induced DNA fragmentation and cytotoxicity. In the study reported here, we further ...
Synthesis of potential antiprogestogens
(Elsevier, 1976-01)
Acylated derivatives of 17[alpha]-hydroxyprogesterone were prepared in order to test the hypothesis that dialkylamino alkyl moieties have the effect of transforming progestogens into antiprogestogens. This approach has ...
Studies on morpholinosphingolipids: Potent inhibitors of glucosylceramide synthase
(Elsevier, 1994-04-25)
Synthetic 1-morpholino-1-deoxyceramides were designed to inhibit glucosylceramide synthase. The most potent inhibitor 2a possesses the unnatural R,R-configuration of D-threo-sphingosine.
The inhibitive effects of steroid analogues in the binding of tritiated 5[alpha]-dihydrotecto3terone to receptor proteins from rat prostate tissue
(Elsevier, 1975-02)
The relationship between molecular structure and the binding potential of steroids to receptor proteins was investigated. Twenty-four selected steroids were studied in minced incubations of rat pro state tissue. Measurements ...
Augmentation of hepatic doxorubicin extraction with extracorporeal filtration avoids the dose-dependent, nonlinear increase in AUC observed with systemic administration
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1997-12)
Purpose : Regional therapy of primary or metastatic liver cancer with low hepatic extraction ratio drugs such as doxorubicin is constrained by development of systemic toxicity. To examine the effect of augmentation of ...
Paired-ion high-performance liquid chromatographic assay for plasma choline
(Elsevier, 1980-03-14)
Choline was isolated from deproteinized plasma by cation-exchange chromatography. Isolated choline was directly converted to the 3,5-dinitrobenzoate derivative and was analyzed by paired-ion high-performance liquid ...
New spectrophotometric and radiochemical assays for acetyl-CoA: Arylamine N-acetyltransferase applicable to a variety of arylamines
(Elsevier, 1985-03)
Simple and sensitive spectrophotometric and radiochemical procedures are described for the assay of acetyl-CoA:arylamine N-acetyltransferase (NAT; EC 2.3.1.5), which catalyzes the reaction acetyl-CoA + arylamine --> ...