Now showing items 3691-3698 of 3698
Engineering the Quantitative PCR Assay for Decreased Cost and Complexity.
(2011)
The quantitative polymerase chain reaction (qPCR) is an assay of target nucleic acid concentration. Clinical applications of quantitative PCR include measurement of HIV viral load, measurement of bacterial infection, and ...
Optimization of RiPCA for the Live-Cell Detection of Pre-MicroRNA-Protein Interactions
(Wiley Periodicals, Inc., 2022-12-16)
Advancements in methods for identifying RNA-protein interactions (RPIs) on a large scale has necessitated the development of assays for validation of these interactions, particularly in living cells. We previously reported ...
Transparent and Self-Healing Elastomers for Reconfigurable 3D Materials
(Wiley Periodicals, Inc., 2022-12)
Transparent soft materials are widely used in applications ranging from packaging to flexible displays, wearable devices, and optical lenses. Nevertheless, soft materials are susceptible to mechanical damage, leading to ...
Identification of regulatory variants of carboxylesterase 1 (CES1): A proof-of-concept study for the application of the Allele-Specific Protein Expression (ASPE) assay in identifying cis-acting regulatory genetic polymorphisms
(Wiley Periodicals, Inc., 2023-01)
It is challenging to study regulatory genetic variants as gene expression is affected by both genetic polymorphisms and non-genetic regulators. The mRNA allele-specific expression (ASE) assay has been increasingly used for ...
Intergenerational arsenic exposure on the mouse epigenome and metabolic physiology
(John Wiley & Sons, Inc., 2023-02)
Inorganic arsenic (iAs) is one of the largest toxic exposures to impact humanity worldwide. Exposure to iAs during pregnancy may disrupt the proper remodeling of the epigenome of F1 developing offspring and potentially ...
Super-Strong Hydrogel Composites Reinforced with PBO Nanofibers for Cartilage Replacement
(Wiley Periodicals, Inc.Imperial College Press, 2023-02)
Cartilage replacement materials exhibiting a set of demanding properties such as high water content, high mechanical stiffness, low friction, and excellent biocompatibility are quite difficult to achieve. Here, ...
BayeStab: Predicting effects of mutations on protein stability with uncertainty quantification
(John Wiley & Sons, Inc., 2022-11)
Predicting protein thermostability change upon mutation is crucial for understanding diseases and designing therapeutics. However, accurately estimating Gibbs free energy change of the protein remained a challenge. Some ...
Machine Learning Guided Drug Discovery: Comprehensive Application in Janus Kinase Inhibitor Design
(2023)
Conventional drug discovery is often plagued by resource-intensive processes, leading to significant attrition rates due to challenges such as efficacy shortcomings, safety concerns and suboptimal pharmacokinetics (PK). ...