Now showing items 4331-4340 of 4346
Intergenerational arsenic exposure on the mouse epigenome and metabolic physiology
(John Wiley & Sons, Inc., 2023-02)
Inorganic arsenic (iAs) is one of the largest toxic exposures to impact humanity worldwide. Exposure to iAs during pregnancy may disrupt the proper remodeling of the epigenome of F1 developing offspring and potentially ...
Super-Strong Hydrogel Composites Reinforced with PBO Nanofibers for Cartilage Replacement
(Wiley Periodicals, Inc.Imperial College Press, 2023-02)
Cartilage replacement materials exhibiting a set of demanding properties such as high water content, high mechanical stiffness, low friction, and excellent biocompatibility are quite difficult to achieve. Here, ...
Structural Analysis of the Helicobacter pylori Pore-Forming Toxin, VacA
(2020)
Helicobacter pylori is a Gram-negative bacterium that persistently colonizes the stomachs of >50% of the human population, with a prevalence as high as 90% in developing nations. H. pylori infection causes gastritis and ...
BayeStab: Predicting effects of mutations on protein stability with uncertainty quantification
(John Wiley & Sons, Inc., 2022-11)
Predicting protein thermostability change upon mutation is crucial for understanding diseases and designing therapeutics. However, accurately estimating Gibbs free energy change of the protein remained a challenge. Some ...
The Reactions of Flavin-Dependent Thymidylate Synthase from Thermotoga Maritima.
(2011)
For decades the biosynthesis of thymidylate was thought to be catalyzed solely by thymidylate synthase. Recently a structurally unrelated new class of thymidylate synthase, ThyX, was found in many bacterial organisms ...
Exploring Novel Enzyme Activity and Genetic Diversity Through Biocatalytic Engineering and Microbiome Mapping
(2021)
Nature provides us with a wealth of natural product molecules and biological catalysts that represent a rich source of new life-saving pharmaceutical medicines and biotechnological tools for green chemistry. The synthetic ...
Understanding the Mechanism of Disaggregation and Unfolding of the hsp100 Family Through cryo-EM Structure Determination
(2022)
Proteins in the cell can become misfolded, leading to toxic aggregates. These toxic aggregates, which can increase during cellular stress and aging, are hallmarks of many neurodegenerative diseases, including Alzheimer’s ...
Structure and Targeting of Precursor MicroRNA-31: From Mechanism to Application
(2024)
As an essential post-transcriptional regulator of gene expression, microRNA (miRNA) levels must be strictly maintained. The biogenesis of many miRNAs is regulated by trans-acting protein partners, which exert control through ...
Investigating the Regulatory Pathways of the Mitotic Oscillator Via a High-Throughput Droplet-Based System
(2024)
This dissertation aims to investigate the properties of the mitotic oscillator and its complex regulatory mechanism. Responsible for the progression of the cell cycle through its various stages, the mitotic oscillator is ...
Mechanistic and Pharmacological Studies of 11 -hydroxylase (P450 11B1) and Aldosterone Synthase (P450 11B2)
(2024)
The cytochrome P450 enzymes, 11β-hydroxylase (P450 11B1), and aldosterone synthase (P450 11B2) carry out the final stages of cortisol and aldosterone synthesis in the adrenal glands. Cortisol, the primary glucocorticoid, ...